NOTE: Only physicians can participate in “hands on” portions. Other healthcare professionals and laboratory staff will observe surgeries only.
DAY ONE
9:00 am – 10:30 am
Lecture: Stem cell biology, cell culture, trypsin, and plating.
10:30 am - 12:30 pm
Stations – 3 groups work under hood to plate stem cells and receive their own sample of already plated cells.
12:30 pm - 1:30 pm
Lunch.
1:30 pm - 5:00 pm
Clinical Experience – Hands-on training with real patients.
DAY TWO
9:00 am – 10:30 am
Lecture: Flow cytometry, gram stain, trypan, potency (differentiation).
10:30 am - 12:30 pm
Harvest and freeze cells. (perform counts and flow).
12:30 pm - 1:30 pm
Lunch.
1:30 pm - 5:00 pm
Stations – 3 groups: Flow cytometry, Gram Stain, Trypan.
DAY THREE
9:00 am – 10:00 am
Lecture: Cryopreservation.
10:00 am - 11:30 am
Lecture: Quality control – Endotoxin, Sterility, Mycoplasma.
11:30 am - 12:30 pm
Lecture: Gowning, Cleanroom maintenance, environmental monitoring.
12:30 pm - 1:30 pm
Lunch.
1:30 pm - 3:30 pm
Stations: Endotoxin - Sterility - Mycoplasma.
3:30 pm - 5:00 pm
Stations: Gowning to enter cleanroom - Calibration and PM - Environmental monitoring.
DAY FOUR
9:00 am – 9:30 am
Stem Cell Biology.
9:30 am - 10:15 am
Embryonic Stem Cells.
10:15 am - 10:45 am
Induced Pluripotent Cells.
10:45 am - 11:00 am
Break.
11:00 am - 12:00 pm
Bone Marrow Derived Mesenchymal Stem Cells.
12:00 pm - 12:30 pm
Fat Collection Procedures.
12:30 pm - 1:15 pm
Lunch.
1:15 pm - 6:00 pm
Patient Demonstration in Clinic.
DAY FIVE
9:00 am – 9:30 am
Platelet Rich Plasma.
9:30 am - 10:45 am
Adipose Stem Cells.
10:45 am - 11:00 am
Break.
11:00 am- 11:30 am
Regulatory Issues.
11:30 am - 12:00 pm
GMP Practices.
12:00 pm - 12:30 pm
Lunch.
12:30 pm - 5:00 pm
Patient Demostration in Clinic.
DAY SIX
9:00 am – 9:30 am
Comparative Colony Counting for Human Samples.
9:30 am - 10:45 am
Manual Colony Identification for Human Samples.
10:45 am - 11:00 am
Break.
11:00 am - 11:30 am
Colony Identification and Counting.
11:30 am - 12:00 pm
Mitosis and differentiation.
12:00 pm - 12:30 pm
Lunch.
12:30 pm - 5:00 pm
Stem cell collection & preservations. Different routes of administration.
DAY SEVEN
9:00 am– 10:15 am
View supplemental videos & information on regenerative medicine.
10:15 am - 10:30 am
Break.
10:30 am - 11:30 am
Pre & Post Medical Instruction.
11:30 am - 12:00 pm
Lunch.
12:00AM - 2:00 pm
Wrap Up Session, Discussion, Quiz, Evaluation, Certificate Presentation.